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1. Fisher sonic dismembrator model 500 manual serial#
2. Fisher sonic dismembrator model 500 manual manual#

Supplementary_files_format_and_content: bigwig files were generated by deeptools. The prepared merged BAM files were analyzed by deeptools to generate bigwig files and normalized values and profiles. Doys in culture There was no loss in viability which was checked by trypan blue exclusion method. tuberculosis in the suspension was ascertained by counts in a Thoma counting chamber. If the duplicates were similar each other, they were merged and sorted by samtools v1.3. to mild sonication using Fisher sonic dismembrator (Model 300) for 30 s twice to disaggregate the clumps. The sorted BAM files of biological duplicates were compared by deeptools. Then, the aligned files were compressed and sorted by samtools v1.3. The qualified fastq files were aligned to Spombe ASMv2 genome fa file using novoalign v3.03.02. The quality of fastq files were checked by fastqc and trimmed by trim_galore

Fisher sonic dismembrator model 500 manual manual#

General Manual of Truseq ChIP library preparation kit. The IP and input DNA were eluted by Quiagen PCR purification kit. Proteins in both IP and input samples were degraded by 2hr 30ug proteinase K rxn and de-crosslinked at 65 Celcius degrees for at least 8hrs. IP samples were washed and eluted by proper buffers in Sigmaprep spin column.

Fisher sonic dismembrator model 500 manual serial#

IP samples were aliqouted from the sonicate and immunoprecipiated by proper amount of antibody and protein A/G beads, washed by ChIP lysis buffer for over-night at 4 Celcius degrees. D100 OEM: Fisher Scientific Modality: Research Lab Serial : FS2507 Cosmetic Condition: Good Functional Condition: Untested Package Type: Small Package Weight: 9. Debris of the sonicate was excluded by centrifugation by 14,800rpm at 4 Celcius degree.

ChIP cells were disrupted by glass bead vortexing in ChIP lysis buffer and sonicated by Sonic Dismembrator Model 500, Fisher Scientific. Cells were harvested by centrifugation at 4 Celcius degrees and the cell pellets were washed by 15mL of pH7.5 TBS buffer per a conical tube. Cell cultures were inoculated with shaking for aeration at proper temperatures.Įvery ChIP-seq cells were grown up to mid-log phase and harvested after final 1% formaldehyde fixation and 125mM glycine quencing. GEO help: Mouse over screen elements for information.Īntibody: Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S2) antibodyįinal 1% formaldehyde fixation and 125mM glycine quencing were performed before cell harvest.Ĭell culture were inoculated twice.